Figure 3From: Smad3 is required for the survival of proliferative intermediate progenitor cells in the dentate gyrus of adult miceSmad3 promotes the proliferation of NPCs in the rostral DG. To analyze the proliferation of progenitor cells, mice received a daily injection of BrdU (100 mg/Kg) over 5 consecutive days and they were sacrificed 2 days after the final injection. (A) Smad3 deficiency did not affect the number of BrdU-ir cells in the SGZ, GCL or the hilus. (B-C) Representation of the SGZ along the rostro-caudal axis showed that Smad3-/- mice had more BrdU-ir cells in the initial rostral 500 μm but not in the middle and caudal regions of the SGZ (*, P ≤ 0.05, Student t-test, n = 5-7 mice per genotype). (D) Confocal images showing Smad3 expression in BrdU labeled proliferative cells of the SGZ. Scale bar 200 μm and 10 μm. (E-F) The endogenous marker of cell proliferation Ki-67 further confirmed the enhanced progenitor cell proliferation in the initial 750 μm rostral portion of the DG in Smad3-/- mice compared to that in Smad3+/+ mice (*, P ≤ 0.05, Student’s t-test, n = 5-6 mice per genotype). (G) The number of Nissl stained neurons in the rostral DG showed a non-statistical trend to increase in Smad3-/- mice in the rostral DG (P = 0.054, Student’s t-test, n = 6 mice per genotype).Back to article page