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Figure 7 | Cell Communication and Signaling

Figure 7

From: C3G forms complexes with Bcr-Abl and p38α MAPK at the focal adhesions in chronic myeloid leukemia cells: implication in the regulation of leukemic cell adhesion

Figure 7

p38α MAPK downregulate the expression and phosphorylation of FA proteins. (A) Representative Western blots showing the expression and phosphorylation (p) of FAK, p130Cas, paxillin (Pax), CrkL, p38α and integrin α5 (Int α5) in K562 clones expressing pSuper-p38α MAPK (p38αi) or the empty vector (pSuper). Cells were cultured in suspension (Susp) or attached to fibronectin (FN). Relative ratios between the levels of the different analyzed proteins and ß-tubulin are shown. (B) The histogram represents the quantification by densitometry of the Western blot bands for each protein, relative to ß-tubulin. Susp: suspension; FN: fibronectin. (C) Western blot showing the expression of p140C3G in K562 cells expressing pSuper-p38α MAPK (p38αi) or empty vector (pSuper) grown on suspension. (D) Representative Western blots showing the expression and phosphorylation (p) of paxillin (Pax), CrkL, p38α and integrinα5 (Int α5) in K562 cells expressing pSuper-p38α MAPK (p38αi) or empty vector (pSuper) grown on suspension and treated or not with 5 μM SB203580 (SB) for 48 h. Relative ratios between the levels of the different analyzed proteins and ß-tubulin or ß-actin are shown.

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Cell Communication and Signaling

ISSN: 1478-811X