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Figure 2 | Cell Communication and Signaling

Figure 2

From: C3G forms complexes with Bcr-Abl and p38α MAPK at the focal adhesions in chronic myeloid leukemia cells: implication in the regulation of leukemic cell adhesion

Figure 2

C3G colocalizes with phospho-p130Cas, Cbl, Abi1 and Bcr-Abl in K562 cells. Confocal microscopy images of K562 cells adhered to fibronectin covered slides (10 μg/ml) and labeled with anti-C3G (G4, mouse monoclonal, rows 1 and 2 or 1008, rabbit polyclonal, rows 3 and 4) and either, anti-phospho-p130Cas (rabbit polyclonal), anti-Cbl (rabbit polyclonal), anti-Abi1 (mouse monoclonal) or anti-Abl (mouse monoclonal) specific antibodies as indicated. Anti-FITC and anti-Cy3 were used as secondary antibodies. Nuclei were labeled with DAPI. Control cells (CT) were incubated with DAPI plus the secondary antibodies. DIC: Differential interference contrast microscopy. p-p130Cas: phospho-p130Cas. The bars represent 10 μm.

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Cell Communication and Signaling

ISSN: 1478-811X