Figure 6

Delivery of anti-RAD51 siRNA to HeLa cells via exosomes. HeLa cells were grown with exosomes loaded with RAD51 siRNA by electroporation (Exo + siRNA electroporated). Non-targeted cells were used as negative control (control) in addition to cells grown with non-electroporated mixture of anti-RAD51 siRNA and exosomes (Exo + siRNA). The introduction of siRNA into exosomes was evaluated using a survival analysis of HeLa cells after 5-7 days of co-culturing with RAD51 siRNA electroporated exosomes as compared to non-electroporated mixture of siRNA and exosomes. The survival of the non-transfected cells was assumed as 100%. Error bars represent the standard error of the mean (SE) of at least three independent experiments.