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Figure 5 | Cell Communication and Signaling

Figure 5

From: Exosomes are natural carriers of exogenous siRNA to human cells in vitro

Figure 5

Exosome-delivered RAD51 siRNA induced accumulation of cells in G2/M phase of cell cycle and resulted in apoptosis of the recipient cells. A, Cell cycle distribution of HeLa cells treated by RAD51 siRNA via exosomes the indicated time. Examination of Hoechst 33342 staining revealed typical cell cycle profiles. It was possible to identify G0/G1 peak, G2/M peak, S phase and dead/apoptotic cells (Ap) as a sub-G1 population. The fraction of cells in each cell cycle phase is indicated at the top. B and C, cytometric analyses of mitochondrial transmembrane potential and plasma membrane integrity fluorochromes after RAD51 depletion. HeLa cells were incubated for 72 h with or without (control) exosomes loaded by RAD51 siRNA. DiOC6(3)/PI double staining flow cytometry analyses (B). Yo-Pro-1/PI double staining flow cytometry dot plots (C). D, the cells incubated with exosomes loaded by RAD51 siRNA were confirmed to be dead or apoptotic by microscopy observation of nuclear fragmentation with the Hoechst 33342 staining.

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