Figure 7

CiaD is required for Erk 1/2-cortactin association. INT 407 cells were infected with a C. jejuni wild-type strain, ciaD mutant, ciaD complemented isolate, or uninfected (control) for 45 min. A. The cell lysates were subjected to immunoprecipitation experiments with an antibody against cortactin, separated by SDS-PAGE and blotted for cortactin, p-cortactin, N-WASP, and pErk 1/2. Whole cell lysates (WCL) were also probed with an α-cortactin antibody to confirm similar inputs. Also shown are the blots of the IgG isotype control IP probed with cortactin, p-cortactin, N-WASP and pErk 1/2 antibodies. B. Band intensity of p-cortactin, N-WASP, and pErk 1/2 were normalized to total cortactin from three independent experiments. The asterisks indicate a significant difference (P < 0.01) compared to the value obtained for the C. jejuni wild-type strain, as judged by one-way ANOVA followed by post-hoc Dunnett’s analysis. N.S. indicates no significant difference.