Figure 8
From: The effects of CA IX catalysis products within tumor microenvironment
Effect of fibroblast CA IX downregulation on tumor cells proliferation. DU145 were labeled with CFDA-SE and then plated in single culture and in coculture with fibroblasts knocked down for CA IX gene or non-silenced, in a 1:2 ratio at 20% O2(A) and 1% O2(B). After 40 hours cells were detached and analyzed by flow cytometry. The data show the proliferation index calculated on an average of four experiments (**, p < 0.05, ***, p < 0.01, ****, p < 0.005). Western blot images show the analysis of CA IX expression in non-silenced or knocked down fibroblasts at 20% O2(A) and 1% O2(B).