Skip to main content
Figure 4 | Cell Communication and Signaling

Figure 4

From: The effects of CA IX catalysis products within tumor microenvironment

Figure 4

Effect of bicarbonate on DU145 tumor cells growth. DU145 were labeled with CFDA-SE and then plated in single culture (A), in coculture with fibroblasts in a 1:2 ratio (C) or both (B). The cells were grown at various pHe in DME supplemented either with 25 mM hepes and 10 mM bicarbonate at 37°C and 5% CO2 or in DME supplemented only with 25 mM hepes at 37°C in CO2/bicarbonate free environment. After 40 hours cells were detached and analyzed by flow cytometry. The data show the proliferation index calculated on an average of four experiments (**, p < 0.05).

Back to article page