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Figure 2 | Cell Communication and Signaling

Figure 2

From: HIF-1α activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells

Figure 2

DMOG strengthens F-actin fibers and supports cell structures within spheroids. (A) DMOG strongly affected orientation of F-actin fibers in migrating cells treated with 1 mM DMOG for 24 h. Compared to control cells, a regular pattern of strong parallel F-actin fibers was observed (PromoFluor phalloidin staining, confocal microscopy). Scale bar: 20 μm. (B) F-actin fibers were still highly irregular when cells were treated with 1 mM DMOG for the last 3 h only (DMOG -3 h), whereas treatment with DMOG for the last 6 h induced alignment of F-actin fibers (DMOG -6 h). Arrows indicate gaps between cells. Scale bar: 20 μm. (C) Cell-cell contacts were visualized by staining of VE-cadherin after spheroids were treated with 1 mM DMOG for 24 h. Scale bar: 20 μm. (D/E) F-actin structures (D) and VE-cadherin (E) within the spheroids were visualized by apotome technique. Cortical F-actin and VE-cadherin formed distinct patterns in DMOG-treated cells. Scale bar: 20 μm.

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