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Figure 1 | Cell Communication and Signaling

Figure 1

From: HIF-1α activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells

Figure 1

DMOG alters cell-cell and cell-matrix interactions. Spheroids of microvascular endothelial glEND.2 cells were generated as described in Materials and Methods. Upon plating on collagen IV-coated plates cells were treated with 1 mM DMOG for 24 h. (A) Radial migration off the spheroids after 24 h was significantly impaired by DMOG-treatment and more cells remained in the residual spheroids. F-actin fibers were visualized with PromoFluor phalloidin, nuclei were stained with Hoechst. Scale bar: 100 μm. (B) For quantification at least 6 spheroids were analyzed and the data of 7 independent experiments were summarized as means ± SEM, **p < 0.01, one sample t-test. C: Control cells; D: DMOG-treated cells; RS: area covered by residual spheroids; Area: area covered by migrated cells; Cells: number of cells migrated off the spheroids.

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