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Figure 2 | Cell Communication and Signaling

Figure 2

From: Up-regulation of COX-2/PGE2 by endothelin-1 via MAPK-dependent NF-κB pathway in mouse brain microvascular endothelial cells

Figure 2

Involvement of ET B receptors in ET-1-induced COX-2 expression. (A) RT-PCR analysis of ET receptor expression in bEnd.3 cells. Lane 1: maker. Lane 2: ETA primers and bEnd.3 RNA. Lane 3: ETB primers and bEnd.3 RNA. (B, C) Cells were pretreated with BQ-123 or BQ-788 for 1 h and then incubated with ET-1 for (B) 6 h and (C) 1 h. (D) Cells were transfected with siRNA for ETB receptor for 24 h and then exposed to ET-1 for 6 h. The (B, D) COX-2 protein and (C) mRNA were analyzed by Western blot and RT-PCR, respectively as described in Figure 1. Data are expressed as mean ± SEM of three individual experiments (n=3 in each group, #P<0.01 as compared with cells stimulated by ET-1 alone).

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