Figure 5
From: A rapid and sensitive assay of intercellular coupling by voltage imaging of gap junction networks
Effect of cell uncoupling by CO 2 . (A) Four selected frames from the same image sequence showing the progressive reduction of the number of cells coupled to the stimulated cell before and during exposure to 100% CO2; the top left image was captured 10.0 s before the delivery of the carrier wave stimulus to cell 1; CO2 delivery started at 25 s and was maintained thereafter; scale bar, 25 μm. (B) Time course of pixel averages from the color−coded ROIs shown in (A). (C) Numerical simulation of the electrical uncoupling process; the effect was mimicked by rapidly decreasing the value of junctional conductance g j (black dashed line) in the network model of Figure 4 from an initial value of 206 nS to 2 nS.