Figure 3
From: A rapid and sensitive assay of intercellular coupling by voltage imaging of gap junction networks
Direct readout of network connectivity by large–scale optical recordings of Vf2.1.Cl fluorescence responses to a 0.5 Hz 35 mV carrier wave. (A) Representative false–color images showing the spatial distribution of Vf2.1.Cl relative amplitude signals in cultures from P5 wild type (top left), connexin30T5M/T5M (top right) and connexin30−/− (bottom left) mice; the bottom right image refers to a wild type culture in which gap junction channels were blocked by 20 min incubation with CBX (100μM); in this image, the area with a residual relative amplitude signal (226 μm2) is very close to the average area of a single cell in this part of the culture (210±7 μm2, n = 10 cells); scale bars, 25 μm. (B) Suprathreshold area distributions shown in box plot form; see main text for details.