Transmigration route of Campylobacter jejuni across polarized intestinal epithelial cells: paracellular, transcellular or both?

Backert, Steffen; Boehm, Manja; Wessler, Silja; Tegtmeyer, Nicole

doi:10.1186/1478-811X-11-72

Cell Communication and Signaling

Table 2 In vitro studies of C. jejuni translocation across polarized epithelial cell lines using transwell assays^a

From: Transmigration route of Campylobacter jejuni across polarized intestinal epithelial cells: paracellular, transcellular or both?

Applied cell model	Time of cell differentiation/growth	Confirmation of proper TJs before infection	C. jejuni strains used	Negative controls used	Applied methods to investigate transmigration	C. jejuni factors involved	Host factors involved	Proposed transmigration route	TER values during transmigration	References
Caco-2	10-14 days	TER (300–500)	78–27, 81116, M129, F38011	E. coli DH5α	TWA, TER, PIS, SEM, TEM	NP	NP	Paracellular and transcellular	Unchanged (within 6 h)	[40]
Caco-2	NP	NP	37 clinical isolates	E. coli DH5α	TWA, CAA, GPA, CTA	NP	NP	Paracellular	NP	[36]
Caco-2	10-14 days	NP	81116	E. coli DH5α	TWA, TER, CAA, GPA, IB	FlaA/B	NP	NP	Unchanged (5 h)	[37]
Caco-2	10-14 days	TER (250)	6 clinical isolates	E. coli DH5α	TWA, TER, CPT	NP	NP	Paracellular and transcellular	Small drop of TER (from 250 to 200), but loss after 24 h	[49]
Caco-2	7 days	TER (430)	10 clinical isolates	NP	TWA, TER, CAA, GPA	NP	NP	Paracellular and transcellular	Changes were strain dependent (6 h)	[38]
T84	NP	NP	81–176, F38011	E. coli MRF	TWA, CAA, GPA	NP	NP	Paracellular	NP	[42]
Caco-2	7 days	TER (>1000)	81–176, NCTC11168	NP	TWA, SEM, TEM, GPA	NP	NP	Paracellular and transcellular	NP	[39]
HCA-7, T84	8-10 days	TER (400–550)	19 clinical isolates	NP	TWA, GPA, TEM, LDH, MF, IL-8, PGE2	NP	NP	Paracellular	Unchanged (12 h)	[50]
Caco-2	17 days	TER (1056)	R27456	E. coli DH5α	TWA, GPA, EPA, PIS	NP	NP	Transcellular	Unchanged (48 h)	[84]
T84, MDCK-I	NP	TER (400–500)	NCTC11168, 81–176, TGH9011	NP	TWA, PIS, GPA, EPA, TEM	FlgF	PI3-K	Transcellular	Unchanged (24 h), drop after 48 h	[43]
T84	NP	TER (values NP)	81–176, CHR213	E. coli HB101	TWA, PIS, IFM	FlaA/B	Cholesterol, caveolin	Transcellular	Unchanged (4 h)	[85]
MKN-28	14 days	TER (130–150)	81–176, NCTC11168	E. coli DH5α	TWA, TER, CA, ECA, IB	HtrA	E-cadherin	Paracellular	Unchanged (24 h)	[44, 45]
Caco-2	19 days	TER (values NP)	GB11, GB19	NP	TWA, IFM, GPA	Cst-II	NP	Transcellular	NP	[59]

^aAbbreviations: CAA (cell adhesion assay), CA (casein assay); CPT (cell permeability test using ¹⁴C-Inulin labelling); Cst-II (sialyltransferase); CTA (cytotoxic activity assay); ECA (E-cadherin cleavage assays), EPA (epithelial permeability assay); FlaA/B (flagellin genes A and B); FlgF (flagellar gene F); GPA (gentamicin protection assay); MF ([³H] mannitol flux); IFM (immunofluorescence microscopy); HtrA (high temperature resistant protein A, a serine protease); IB (immunoblotting ); IL-8 (IL-8 measurement); LDH (lactate dehydrogenase test); NP (not provided in the study); PGE 2 (prostaglandin E2 measurement by ELISA); PI 3-K (phosphoinositid-3-kinase); PIS (pharmacological inhibitor studies); SEM (scanning electron microscopy); TEM (transmission electron microscopy); TER (transepithlial electrical resistance measurement, values given in Ohms per cm²); TJ (tight junction); TWA (transwell assay).

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ISSN: 1478-811X

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