Skip to main content
Figure 7 | Cell Communication and Signaling

Figure 7

From: Knockdown of the C. elegans Kinome identifies Kinases required for normal protein Homeostasis, Mitochondrial network structure, and Sarcomere structure in muscle

Figure 7

Systematic identification of kinases required for normal sub-cellular processes in muscle. Three parallel screens using RNAi feeding clones against 397 kinase-encoding genes were performed. Screens were run in parallel on the same genes, typically 20 per week. In each screen, all genes were assessed for the effect of RNAi upon a single sub-cellular process within muscle (using a different transgenic reporter in each screen). (A) All genes were assessed for the effect of chronic, multi-generational RNAi knockdown. PD55 was used for the proteostasis screen, CB5600 for the mitochondrial network structure screen, and PJ727 for the sarcomere structure screen. Upon reaching adulthood, F1 or F2 progeny were observed on two consecutive days using light microscopy after staining for β-galactosidase in the proteostasis screen or epifluorescence microscopy for the mitochondrial or sarcomere screens. (B) In each screen, identified genes and treatments that resulted in a lack of progeny were assessed for the effect of acute, single generation RNAi knockdown in age synchronized adults. Adult worms were observed using light microscopy after staining for β-galactosidase in the protein degradation screen or epifluorescence microscopy for the mitochondrial or sarcomere screen (to confirm normal baselines) and then allowed to grow on RNAi plates for an additional 72 hours. Additional measurements were taken 24*, 48, and 72 hours after being placed on RNAi plates. *24 hour measurements were only taken in the protein degradation screen.

Back to article page