Figure 2

Kinases that appear to be required for normal mitochondrial network structure. (A) Sample images of GFP labelled mitochondrial networks, and nuclei (large over exposed circles), showing normal mitochondrial network structure in control, non-RNAi treated, CB5600 animal (left) and RNAi treated animals showing disrupted network structure. RNAi treatments are indicated in the lower right corner. Scale bars represent 20 μm. (B) Kinases identified as required for normal maintenance of mitochondrial network structure in adult muscle. Indicated RNAi treatments were conducted chronically and followed up acutely and scored for loss of mitochondrial network organization, see materials and methods. Displayed are scoring data for each time point (n = 20-30). Green indicates networks not appreciably different from controls. Red indicates that at least 25% of scored animals displayed disrupted mitochondrial network structure versus controls. Dark red indicates that at least 50% of scored animals displayed disrupted mitochondrial network structure versus controls. (C) Kinases identified as required for normal mitochondrial network structure. RNAi treatments were conducted and scored as in (B) however the listed treatments produced a defect in the chronic screen but not the acute screen. – Indicates lack of data for a given time point. † Indicates a treatment was classed as producing a lack of progeny in this screen.