Figure 1

Kinases that appear to be required for normal muscle protein synthesis and/or degradation. (A) Sample images of normal β-galactosidase reporter staining (dark blue) in control, non-RNAi treated, PD55 animal (left) and RNAi treated animals showing decreased β-galactosidase reporter staining. RNAi treatments are indicated in the lower right corner. Scale bars represent 100 μm. (B) Kinases identified as required for normal lack of protein degradation in muscle. Indicated RNAi treatments were conducted chronically and followed up acutely and scored for decreased β-galactosidase reporter staining, see materials and methods. Displayed are scoring data for each time point (n = 20-30). Green indicates staining not appreciably different from controls. Red indicates that at least 25% of scored animals displayed lighter/diffuse blue or lack of blue staining versus controls. Dark red indicates that at least 50% of scored animals displayed light/diffuse blue or lack of blue staining versus controls. (C) Kinases identified as required for normal protein homeostasis in muscle. RNAi treatments were conducted and scored as in (B) however the listed treatments produced a defect in the chronic screen but not the acute screen. – Indicates lack of data for a given time point. ‡ Indicates a treatment was classed as producing a lack of progeny in this screen.