Figure 4
From: Hierarchical scaffolding of an ERK1/2 activation pathway
IQGAP1 is required for activation of cytoskeletal ERK1/2. (A) IQGAP1 knock down interferes with filamentous localization of phospho-ERK1/2. A7r5 cells on coverslips transfected with siRNA directed against IQGAP1 were stimulated with DPBA or FCS. After fixing, cells were co-stained for IQGAP1 (red channel) and phospho-ERK1/2 (green channel); filamentous actin was stained with Alexa350-phalloidin (blue channel). The panels show representative images from one out of three independent experiments. (B) The same images as in (A) shown in black and white for enhanced contrast. Scale bar, 20 μm.