Figure 1

β2AR-PDE4D interaction in the heart. Co-immunoprecipitation (co-IP) followed by western blotting in cardiac extracts from normal (sham) B1KO and βARKct/B1KO (CT/B1KO) mice to measure the β₂AR-PDE4D interaction in the heart. Representative immunoblots are shown in (A), and the amounts of the co-immunoprecipitated PDE4D isoforms, as measured by densitometry and normalized with the amount of β₂AR pulled down in the co-IP, are shown in (B). *, p<0.05, vs. B1KO; n=4 independent experiments (i.e. performed on 4 different hearts from each mouse line). IP: Immunoprecipitation, IB: Immunoblotting.