Figure 6
From: Cold shock Y-box protein-1 proteolysis autoregulates its transcriptional activities
The carboxy-terminal YB-1 protein fragment (CTF) regulates transcriptional activity. A. Transcriptional activity of CTF on the MMP2 promotor construct pGL2MMP-2/RE-1. Rat mesangial cells were transfected with equal amounts of DNA (as indicated). After 48 hours cell lysates were prepared and luciferase assays were performed. All experiments were performed in triplicates and confirmed in three independent experiments. B. Transcriptional activity of the CTF on the Col1a1 promotor construct pGLα1-2.3. Rat mesangial cells were transfected with the indicated expression vectors and incubated for 48 hours. Luciferase assays were performed thereafter using cell lysates. All experiments were performed in triplicates and confirmed in three independent experiments.