Figure 6From: Cucurbitacin covalent bonding to cysteine thiols: the filamentous-actin severing protein Cofilin1 as an exemplary targetCofilin1 knockdown does not affect F-actin structures. A) Western blotting of MCF7 lysates indicating efficient siRNA-mediated Cofilin1 knockdown but no effect of non-targeting control (NTC) siRNA. B) Immunofluorescence of F-actin and Cofilin1 following transfection with NTC or Cofilin1 siRNA revealed that Cofilin1 inhibition is not sufficient to induce significant F-actin changes.Back to article page