Figure 1

Cytotoxicity of cucurbitacin compounds. A) Chemical structures of cucurbitacin D, E and I. B) Cytotoxicity dose–response profiles for cucurbitacin D, E and I. Viable MCF7 breast cancer cell numbers were determined by counting DAPI stained nuclei 3 days after cucurbitacin treatment at doses from 0.3 nM to 10 μM with means and SEM depicted for 9 replicates. C) Relative single cell fluorescence intensity of phalloidin stained F-actin measured 4 h after cucurbitacin addition at doses from 0.3 nM to 10 μM. Means and SEM are depicted for 3 replicates.