Kinetic schemes and model simulations for motifs 1–3. (a-c) Schematic kinetic diagrams of the network motifs 1–3 described in the text. (d) Comparative temporal dynamics of the active output level for the three motifs (e, f) Comparative temporal dynamics of the active output at increasing Kinase abundance for motif 1 and 2, respectively. Parameter values used: high [Kinase] = 1000 nM, medium [Kinase] = 100 nM, low [Kinase] = 10 nM. Detailed description of the models is given in the Additional file1 (SI) document, along with the remaining parameter values.