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Figure 1 | Cell Communication and Signaling

Figure 1

From: Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling

Figure 1

Effects of Rho, ROCK, MLC signaling inhibition on the invasiveness and morphology of A3 rat cells. (A) Immunodetection of GFP-tagged RhoA and MLC proteins in A3 cells. (B) The in vitro invasion of A3 cells in 3D collagen. Treatment of A3 cells with metalloproteinase inhibitor GM6001 has no significant effect on invasion. Inhibition of ROCK by Y-27632 and inhibition of non-muscle myosin II ATPases activity by Blebbistatin in A3 cells as well as inhibition of both RhoA in A3dnRhoA cells and MLC in A3dnMLC cells leads to decreased ability of these cell lines to invade 3D collagen. (C) Morphology of A3 cells in 3D collagen in vitro. Activity of RhoA and ROCK is required for rounded morphology of the A3 cell line. Inhibition of ROCK by Y-27632 and inhibition of RhoA in A3dnRhoA lead to the amoeboid-mesenchymal transition in 3D collagen. Inhibition of metalloproteinases by GM6001, inhibition of non-muscle myosin II ATPases activity by Blebbistatin in A3 cells and inhibition of MLC activity in A3dnMLC cells has no significant effect on morphology.

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