Figure 6
AITC inhibits tumor growth and induces apoptosis in U937 xenografts animal model. 40 nude mice were inoculated with U937 cells (2 × 106 cells/mouse, s.c.) and randomly divided into two groups (20/group) for treatment with AITC (50 mg/kg, i.p., daily, five times per week) or with vehicle control solvent as described in Methods. Tumor growth was measured once a week, and tumor volume (V) was calculated as V = (L × W2) × 0.5, where L is the length and W is the width of a xenograft. (A) Comparison of animal survival of AITC-treated group and vehicle-treated group (P < 0.01). (B) Gross appearance of two mice with solid tumor of vehicle control group and AITC-treatment group. (C) Average tumor volume in vehicle control mice and mice treated with 50 mg/kg AITC. Error bars represent means ± SD. *P < 0.05 or **P < 0.01 compared with vehicle control. (D) Body weight changes of mice during the six weeks of study. Statistical analysis of body weight changes showed no significant differences between AITC treatment and vehicle control groups. (E) After treatment with AITC (50 mg/kg), tumor tissues were sectioned and subjected to H&E staining, TUNEL assay, and immunohistochemistry for evaluating histological morphology, apoptosis and expression of C-Caspase-3 and p-cofilin.