Diet-induced obesity increased pan-PTP activity and DEP-1 activity in liver and skeletal muscle in mice. A-C: Pan-PTP activity was determined in liver, skeletal muscle and adipose tissue by measuring dephosphorylation of a 32P labeled phosphopeptide in total lysates from mice fed an LFD or HFD for 16 weeks. Pan-PTP activity in mice fed with LFD were set to 100%; (n = 10 per group). D-F: Dephosphorylation of a 32P labeled phosphopeptide was measured for precipitated PTP1B and DEP-1 in LFD and HFD mice. Tissue lysates were subjected to immunoprecipitation. PTP1B and DEP-1 activity in mice fed with LFD were set to 100%; (n = 5–6 per group). *P < 0.05, ***P < 0.001.