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Figure 2 | Cell Communication and Signaling

Figure 2

From: Insulin receptor membrane retention by a traceable chimeric mutant

Figure 2

Insulin binding assays. A. Strategy for insulin binding and QD655 labeling. B. HeLa cells expressing Mut-GFP were incubated with 50 nM BAC-Ins and 1 nM QD655, fixed and imaged by confocal microscopy. C. Insulin binging after ACP labeling. D-E. Cells expressing the mutants were labeled with 0.2 μM ACP-S and 1 μM fluorescent CoA and then with 50 nM BAC-Ins and 1 nM QD655. Imaging was performed by confocal (D) or wide field microscopy (E). Scale bars: 10 μm.

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