Figure 6

Immunofluorescence images of co-localization of Cx43, c-Src and IκB-α in GMCs. GMCs were incubated in normal glucose (NG; 5.5 mmol/L) or high glucose (HG; 30 mmol/L) for 30 min. (A (a)) Confocal microscopy was used to evaluate the localization of Cx43 under normal glucose or high glucose conditions. Green fluorescence indicates localization of Cx43. Red fluorescence indicates ZO-1, which served as a cytomembrane marker. Blue fluorescence indicates nuclei. (A (b)) Confocal microscopy was used to evaluate the localization of c-Src under normal glucose or high glucose conditions. Red fluorescence indicates c-Src. Green fluorescence indicates localization of ZO-1. Blue fluorescence indicates nuclei. (B (a)) Confocal microscopy was used to evaluate the localization of Cx43 and c-Src under normal glucose or high glucose conditions. A significant decrease in Cx43 was observed after high glucose treatment. c-Src dissociated from Cx43 and translocated into the cytoplasm after high glucose treatment. Green fluorescence indicates localization of Cx43. Red fluorescence indicates c-Src localization. Blue fluorescence indicates nuclei. (B (b)) Confocal microscopy was used to evaluate the localization of c-Src and IκB-α. c-Src was translocated into the cytoplasm from the cytomembrane after high glucose treatment and then co-localized with IκB-α in the cytoplasm of GMCs. Green fluorescence indicates localization of IκB-α. Red fluorescence indicates c-Src localization. Blue fluorescence indicates nuclei. Scale bar represents 20 μm (magnification 630×).