c-Src activity is upregulated in diabetic kidneys and high glucose-induced GMCs. (A, B) c-Src activity in kidneys of db/db mice and STZ-induced diabetic rats was detected by immunoblotting. (C) c-Src activity in GMCs was measured by immunoblotting for phosphorylation of Tyr416 on c-Src after treatment for 30 min with high glucose (30 mmol/L) and reprobed with an anti-c-Src antibody as a loading control. Mannitol (30 mmol/L) was used as an osmotic control. Experiments were performed at least three times with similar results. *P<0.05 vs. control group.