Figure 6

Experimental (A-D; ref. [28]) and simulated (E-H) flash responses showing that R* shutdown only becomes rate-limiting when RGS activity is greatly increased. When RK is underexpressed 0.4x against a WT background (B, F), the response shows a slight slowing in recovery time compared to WT (A, E). When RGS is overexpressed six-fold against a WT background (C, G), recovery is significantly accelerated. Finally, when RK is underexpressed against a background of RGS overexpression, the WT dynamics are partially recovered. Simulated stimulus intensities were adapted from ref. [28] (see Methods). WT (E) stimuli were 3.2, 13.6, 34.4, 127.2, 362.4 and 896 R* per flash. 0.4x RK underexpression (F) stimuli were 3.2, 13.6, 34.4, 127.2, 362.4, 690.4 and 1496.8 R* per flash. 6x RGS overexpression (G) stimuli were 13.6, 34.4, 127.2, 362.4, 516.8, 694.4, 896 and 1496.8 R* per flash. 0.4x RK UX/6x RGS OX double mutant stimuli were 3.2, 13.6, 34.4, 127.2, 362.4 and 896 R* per flash. (Panels A-D copyright © 2010 Chen et al.).