Figure 10

The evolution of the summed concentrations of several classes of molecular species during the first four seconds of a response to a saturating flash stimulus (118,000 R*). Black traces are simulated with normal Arr oligomerization while red traces are from simulations with Arr oligomerization disabled. Solid traces are simulated with normal RK expression while dashed traces are simulated with 2.4x RK overexpression. A) R* with zero to three phosphates attached. B) R* with zero to three phosphates and bound to Arr. Notice that when Arr cannot form homo-oligomers, it binds to sparsely phosphorylated R* more rapidly and at higher quantities. C) R* with four to six phosphates. When Arr oligomerization is disabled, fewer R* reach this state. D) R* with four to six phosphates and bound to Arr. When Arr oligomerization is disabled, heavily phosphorylated R* is bound by Arr quicker, an effect which is particularly influenced by RK expression levels. E) Monomeric Arr quantities begin to be depleted upon R* activation but they are quickly replenished by the oligomer stores. When Arr oligomerization is disabled, monomeric Arr quantities decrease by a much greater amount and do not recover. F) Arr molecules present in homo-oligomeric forms gradually decrease in quantity, replenishing the available monomer, and do not recover in quantity within the simulated time range.