Skip to main content

Table 1 Process to define which integrins repress CNTF

From: Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression

Integrin

FG

TSP

COL

LAM

VTN

FN1

Leukocyte

Antibody

α1β1

  

i, ii, iii

i, ii, iii

   

β1

α2β1

 

i, iii

i, ii, iii

i, ii, iii

 

ii

 

β1

α3β1

 

i, ii, iii

 

i, iii

 

ii

 

β1

α4β1

 

i, iii

   

i, ii, iii

X

β1

α4β7

     

i, ii, iii

X

 

α5β1

ii

iii

   

i, ii, iii

 

β1

α6β1

 

iii

 

i, ii, iii

   

α6, β1

α6β4

   

i, ii, iii

   

β6

α7β1

   

i, ii, iii

   

β1

α8β1

     

i, ii, iii

 

β1

α9β1

      

X

β1

α10β1

  

i, iii

    

β1

α11β1

  

i, ii, iii

    

β1

αvβ1

    

ii

i, ii, iii

 

β1

αvβ3

i, ii, iii

i, ii, iii

 

ii

i, ii, iii

i, ii, iii

  

αvβ5

    

i, ii, iii

ii

 

αv, β5

αvβ6

     

i, ii, iii

 

αv

αvβ8

     

ii

 

αv

αdβ2

    

iii

iii

X

 

αLβ2

      

X

 

αmβ2

i, ii, iii

     

X

 

αxβ2

i, ii, iii

 

iii

   

X

 

αEβ7

      

X

 

αIIbβ3

i, ii, iii

i, ii, iii

  

i, ii, iii

i, ii, iii

  
  1. We applied the following process to help us to decide which subunit blocking antibodies to test and which integrins most likely repress CNTF. When “italics” appears in a row, that specific integrin is considered unlikely to be involved in repressing CNTF. We first used the data from the ECM substrate experiment (Figure 1A) and the known integrin binding partners to eliminate potential integrins involved in repressing CNTF. Thus, fibrinogen (FG), thrombospondin (TSP), and collagen (COL) had no significant effect on CNTF mRNA levels in C6 cells, as indicated by “italics” in the row of their known integrin partners. The numbers in the cells indicate the following articles that list the specific ligand – integrin relationship: i) Humphries et al., [34] ii) Plow et al. [38], and iii) Takada et al., [39]. Laminin (LAM), vitronectin (VTN), and fibronectin (FN) significantly reduced CNTF expression, as indicated by “bold” numbers in the respective columns. Next, to further help with antibody selection, we eliminated all leukocyte-specific integrins [21]) as they are not present on glial cells (second to last column). Lastly, the results from the antibody experiment (Figure 1B) were used to eliminate a number of β1- and α6-containing integrins (“italics” letters in the last column), leaving three candidates which potentially repress CNTF (“bold” letters in the last column).