Figure 5

FAK inhibition induces CNTF expression in the adult CNS in vivo . A) Intracerebral injection of the FAK inhibitor PF573228 directly in the striatum of mice results in increased CNTF protein 4 hours later as shown in western blots. FAK activity was greatly decreased as shown by lack of phosphorylation. (pFAK). α-tubulin = internal control. Lanes are from individual animals. B) Similar results were obtained after direct injection into the spinal cord. C) Systemic intraperitoneal injections of the FAK inhibitors PF573228 or FAK14 induce CNTF mRNA in the spinal cord or SVZ relative to vehicle only injected mice (Ctrl). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are fold change compared to control (Ctrl) and are means +/− SEM from 3–4 mice per group.