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Figure 3 | Cell Communication and Signaling

Figure 3

From: Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission

Figure 3

Experimental design during the MASER-12 mission. For the analysis of resting T cells three different gravity conditions were tested: μg samples which were installed on the μg Rack in the LAU on board of the rocket, 1g samples that were installed on the on-board reference centrifuge providing 1g, and H/W samples that were installed in the hardware ground module analogue to the flight hardware. With re-entry of the payload into the Earth’s atmosphere all samples were automatically fixed. The analysis of T cells activation involved five different conditions. In addition to the μg and the 1g samples, baseline (BL) samples were used, which were fixed before onset of microgravity (μg phase). At this stage, the μg samples and the 1g samples were activated. The fixation of those two conditions were executed parallel with the appropriate H/W samples, although these were not activated. The fifth condition consisted of culture control (CC) samples, where T cells were cultured under normal culture conditions. Those samples were fixed manually a few minutes after rocket launch due to safety reasons.

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