Figure 6
From: The role of LPA and YAP signaling in long-term migration of human ovarian cancer cells
YAP-dependent amphiregulin (AREG) production/secretion mediated EGFR-dependent LPA-induced cell migration. A, The effects of AG1478 (1 μM, 1 hr pretreatment) and PD153035 (4 μM, 1 hr pretreatment) on LPA-dpYAP (a), or cell migration (b); AREG (100 pg/mL) induced AG1478 (1 μM, 1 hr)-sensitive migration of OVCA433 cells (c). * P < 0.05. B, Basal and LPA (10 μM)-induced AREG in conditioned media (CM) of OVCA433 cells (a). mRNA level of AREG was increased by LPA (b). LPA (10 μM, 8 hr)-induced AREG in CM was YAP- and LPA3-, but not LPA1-dependent (c). LPA-induced AREG production/secretion was blocked by dn-G13, dn-RhoA, but not PTX (100 ng/mL, 16 hr) or dn-Gq (d and e). C, LPA-induced short-term migration (4 hr) was not dependent on YAP or transcription (ActD, 1 μg/mL, 1 hr pretreatment), but was sensitive to PTX (100 ng/mL, 16 hr), LY294002 (10 μM, 1 hr pretreatment) and dn-Rac1 transfection (a). LPA-induced long-term cell migration was sensitive to ActD and CHX treatment (20 μg/mL, 1 hr pretreatment), as well as PTX and LY294002 (b). Data are from three independent experiments (means ± SD). * P < 0.05, ** P < 0.01. D, Summary the LPA-YAP signaling pathway revealed in this work (OVCA433 cell line, left panel) and the previous work [1] (HEK293A cell line, right panel).