, but not or to lesser extent LPA
, and LPA
, mediated the LPA-dpYAP effect. A, OVCA433 (a) and OVCAR5 (c) cells were starved and pretreated with Ki16425 (10 μM) for 1 hr prior to treatment with LPA (10 μM, 2 hr). pYAP was analyzed by Western blot. (b) The effect of Ki16425 on LPA (10 μM, 2 hr)-induced YAP nuclear translocation in OVCA433 cells. Green: YAP; red: DAPI. Representative results are shown. B, (a) The mRNA levels of LPA receptors after siRNA-treatment in OVCAR433 cells were determined by quantitative real-time PCR. Normalized expression values are given as percentage of control siRNA treated samples (means ± SD of three independent experiments). *** P < 0.001. (b) LPA (10 μM, 2 hr)-induced dpYAP effects were determined in LPA receptor specific siRNA-treated cells (48 hr post-transfection). (c) Quantitation of Western blots from (b) presented as fold decrease of pYAP after LPA stimulation compared to unstimulated controls. The data are means ± SD from three independent experiments. * P < 0.05. C, D and E, Cells were pretreated with PTX (100 ng/mL, 16 hr) or transfected with different dn plasmids for 48 hr, starved and then treated with LPA (10 μM, 2 hr). Cell lysates were analyzed by Western blot. Representative results are shown.