LPA-induced dp-YAP and YAP nuclear translocation were dependent on Rho/ROCK, but independent of PI3K, MEK, p38, and AKT. After starved from FBS for 16 hr, cells were pretreated with different inhibitors, SB203580 (10 μM), LY294002 (10 μM), Ki16425 (10 μM), MK2203 (1 μM), Y27632 (10 μM), PD98059 (30 μM) for 1 hr, and C3 (1 μg/mL) for 2 hr prior to stimulation with LPA (10 μM, 2 hr). pYAP expression in OVCA433 (A) and OVCAR5 (B) cells was analyzed by Western blot. C, OVCA433 cells were treated as described in A. Cells were fixed and stained for YAP (green). Cell nuclei were stained with DAPI (red). Representative results are shown.