LPA-induced dpYAP and YAP nuclear translocation in EOC cells. OVCA433 (A) and OVCAR5 (B) cells were starved for 16 hr, then treated with LPA (10 μM) for different times or with different concentrations of LPA for 2 hr. Western blots analyses and quantification methods were described in Materials and Methods. Representative results are shown from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. C, LPA (10 μM, 2 hr)-induced dpYAP was tested in two more EOC cell lines. D and E, LPA-induced YAP nuclear translocation is shown in OVCA433 and OVCAR5 cells. Green: YAP; red: DAPI. F, LPA (10 μM) time-dependently induced de-phosphorylation of TAZ.