Figure 6

Imatinib and dasatinib sensitive Gab2 protein interactions. Scatter plot of proteins showing significant change in the interaction with Gab2 with either (A) similar or (B) different responses to the imatinib and dasatinib treatments. (C) Network diagram of protein-protein interactions of the identified Gab2 interactors. Blue nodes indicate proteins with a similar response to the treatments, green and red nodes are proteins that dissociate stronger in response to Dasatinib or Imatinib, respectively. Red edges indicate protein-protein interactions present in the STRING database (score >0.7) and blue edges indicate putatively novel interactions. Barplot of the observed ratios for (D) Bcr-Abl, (E) PI3 kinases, (F) Casein kinases and (G) 14-3-3 protein isoforms, error bars indicate standard deviation of the two biological replicates. (H) Significantly depleted proteins highlighted in panels (A and B) are tested on their sensitivity to different dasatinib concentrations (0.01 and 1 μM).