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Figure 4 | Cell Communication and Signaling

Figure 4

From: Platelet-derived growth factor-induced Akt phosphorylation requires mTOR/Rictor and phospholipase C-γ1, whereas S6 phosphorylation depends on mTOR/Raptor and phospholipase D

Figure 4

PDGF-BB-induced Erk1/2 signalling affects the kinetics of S6 phosphorylation. NIH3T3 cells (A, B, D, E, F and G) and Rictor-null MEFs (C) were serum-starved for 24 h and then stimulated with PDGF-BB (20 ng/ml) in the absence or presence of CI-1040 (0.5 μM) and rapamycin (10 nM) for 1 h, 1-butanol (1-B, 0.3%), 2-butanol (2-B, 0.3%), BAPTA-AM (10 μM) and EDTA (2 mM) for 30 min, and PMA (1 μM) for 24 h, as indicated. The levels of phosphorylation of Erk1/2, mTOR, Akt and S6, as well as the total protein, were assayed by immunoblotting (Ib) of total cell lysates.

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Cell Communication and Signaling

ISSN: 1478-811X