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Figure 2 | Cell Communication and Signaling

Figure 2

From: Platelet-derived growth factor-induced Akt phosphorylation requires mTOR/Rictor and phospholipase C-γ1, whereas S6 phosphorylation depends on mTOR/Raptor and phospholipase D

Figure 2

PDGF-BB-mediated S6 phosphorylation is regulated by PLD/Ca2+signaling. NIH3T3 cells (A, B) and Rictor-null MEFs (C) were serum-starved for 24 h and then stimulated for indicated time periods with PDGF-BB (20 ng/ml) with or without pretreatment with 1-butanol (1-B, 0.3%), 2-butanol (2-B, 0.3%), and Ca2+ chelators BAPTA-AM (BA, 10 μM) or EDTA (ED, 2 mM) for 30 min, as indicated. Total cell lysates (TCL) were prepared, and the levels of Akt phosphorylation at S473 and T308, S6 phosphorylation, as well as the total protein expression, were assayed by immunoblotting (Ib). The relative protein phosphorylations were quantified for a representative experiment.

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Cell Communication and Signaling

ISSN: 1478-811X