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Figure 1 | Cell Communication and Signaling

Figure 1

From: Platelet-derived growth factor-induced Akt phosphorylation requires mTOR/Rictor and phospholipase C-γ1, whereas S6 phosphorylation depends on mTOR/Raptor and phospholipase D

Figure 1

PDGF-BB-mediated S6 phosphorylation does not require Akt phosphorylation. NIH3T3 cells (A, B, D) and Rictor-null MEFs (C) were serum-starved for 24 h and then stimulated for indicated time periods with PDGF-BB (20 ng/ml) without or with pretreatment for 1 hours (otherwise specified) in the presence of the inhibitors NPV-BKM120 (A), rapamycin (Rap, 10 nM) ( B ), or tricribine (20 μM) (D) for indicated time periods. Total cell lysates (TCL) were prepared, and the levels of Akt phosphorylation at S473 and T308, as well as S6 phosphorylation and the expression of total protein were assayed by immunoblotting (Ib). The relative protein phosphorylations were quantified for a representative experiment.

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