Table 2 Overview of primers used for qRT-PCR analysis
Target gene | Forward sequence | Reverse sequence |
|---|---|---|
ACTA2 | CTGTTCCAGCCATCCTTCAT | TCATGATGCTGTTGTAGGTGGT |
CCL18 | ATGGCCCTCTGCTCCTGT | AATCTGCCAGGAGGTATAGACG |
CCL2 | AGTCTCTGCCGCCCTTCT | GTGACTGGGGCATTGATTG |
CCL7 | ATGAAAGCCTCTGCAGCACT | TCTGTAGCAGCAGGTAGTTGAAGT |
CD14 | AGCTAAAGCACTTCCAGAGC | AGTTGTGGCTGAGGTCTAGG |
CD40 | GGTCTCACCTCGCTATGGTT | CAGTGGGTGGTTCTGGATG |
CD68 | GTCCACCTCGACCTGCTCT | CACTGGGGCAGGAGAAACT |
CLEC10A | AGGGTTTCAAGCAGGAACG | AGGTGTGCCTTCTGCGTAGT |
COL1A1 | GCCTCAAGGTATTGCTGGAC | ACCTTGTTTGCCAGGTTCAC |
COL3A1 | CTGGACCCCAGGGTCTTC | CATCTGATCCAGGGTTTCCA |
GAPDH | AGCCACATCGCTCAGACAC | GCCCAATACGACCAAATCC |
IL1B | TACCTGTCCTGCGTGTTGAA | TCTTTGGGTAATTTTTGGGATCT |
IL1R2 | TTTCTGCCTTCACCCTTCAG | GGCACCTCAGGGCTACAG |
IL6 | ACTTGCCTGGTGAAAATCAT | CAGGAACTGGATCAGGACTT |
MMP1 | GCTAACCTTTGATGCTATAACTACGA | TTTGTGCGCATGTAGAATCTG |
MMP14 | TACTTCCCAGGCCCCAAC | GCCACCAGGAAGATGTCATT |
MMP2 | CCCCAAAACGGACAAAGAG | CTTCAGCACAAACAGGTTGC |
MMP3 | CAAAACATATTTCTTTGTAGAGGACAA | TTCAGCTATTTGCTTGGGAAA |
MRC1 | ACACCAAAACCTGAGCCAAC | CCACCCATCTTCAGTAACTGGT |
TAGLN | CTGTTCCAGCCATCCTTCAT | TCATGATGCTGTTGTAGGTGGT |
TIMP1 | GAAGAGCCTGAACCACAGGT | CGGGGAGGAGATGTAGCAC |
YWHAZ | GATCCCCAATGCTTCACAAG | TGCTTGTTGTGACTGATCGAC |