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Figure 4 | Cell Communication and Signaling

Figure 4

From: Cell plasticity in wound healing: paracrine factors of M1/ M2 polarized macrophages influence the phenotypical state of dermal fibroblasts

Figure 4

CM from M1 macrophages induces dermal fibroblasts with extracellular matrix degradation properties. HDFs upregulated the gene expression of MMP1, MMP2, MMP3, MMP14 and TIMP1 after stimulation with CM of M1 polarized macrophages compared to M2 polarized and unstimulated macrophages (A). HDFs stimulated with CM of M1 macrophages secreted significantly more MMP1, MMP2 and MMP3 protein compared to fibroblasts stimulated with CM of M2 macrophages or unstimulated macrophages (B). HDFs stimulated with M1 CM showed a higher net proteolytic activity compared to HDFs stimulated with CM of M2 and unstimulated macrophages (C). ** p < 0.01, Difference between HDFs stimulated with CM of M1 polarized and CM of M2 polarized macrophages, *** p < 0.001. ## p < 0.01, Difference between HDFs stimulated with CM of M1 polarized and CM of unstimulated macrophages, ### p < 0.001. Gene expression analysis data were analyzed using two-way ANOVA followed by Bonferroni’s post-test, n = 4. MMP secretion was analyzed using one-way ANOVA followed by Tukey’s post-test, n = 3. Proteolytic activity was analyzed using one-way ANOVA followed by Tukey’s post-test, n = 4.

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