Skip to main content
Figure 5 | Cell Communication and Signaling

Figure 5

From: PAG/Cbp suppression reveals a contribution of CTLA-4 to setting the activation threshold in T cells

Figure 5

CTLA-4 suppression restores the defect in proliferation caused by PAG suppression. Primary human T cells were transfected with siRNAs targeting Renilla (ctrl), PAG (PAGsi), CTLA-4 (CTLA4si) or PAG + CTLA-4 (PAGsi+CTLA4si). Cells were then stimulated with indicated stimuli for three days on a plastic plate. (A) To demonstrate the downregulation of protein expression by siRNAs, total cell lysate staining for PAG and CTLA-4 is shown. (B) The proliferation was measured by 3H-Thymidin incorporation at 72 hours. Mean value of triplicates (±SD) is depicted. Data were analyzed by the Student’s t-test (*, P<0.05; **, P<0.01). Data are representative of three independent experiments. (C) Primary human T cells transfected with the siRNAs indicated were stimulated with CD3 antibodies for the indicated time, lysed and subjected to Western blotting. The activation of ZAP70 and PLCg was determined by phospho-specific antibodies. Actin is shown for equal loading. Quantification of the blots can be found in the supplemental material (Additional file 8: Figure S8).

Back to article page