Figure 2

Selected microscopy applications in systems biomedicine: (A), (B), and (C) Analysis of mitotic events by hidden Markov modeling to evaluate mitotic phase transitions [33]. (A) Trellis diagram showing class prediction estimates for a given cell. (B) Event order map and example of time-series images. (C) Double-stained HeLa cells in different cell division states. (D) and (E) Nematode morphology analysis. (D) Automated segmentation of single worms [34]. (E) Straightening of single nematode datasets [35]. (I) and (J) Body atlas for zebrafish [36]. (I) TH-expressing zones are highlighted in green. (J) Registration of single image information into a zebrafish body-atlas database. (F), (G), and (H) In vivo imaging of mice. (F) Miniaturized microscope weighing 1.9 g [37]. (G) Dynamic analysis of the intestinal mucosal barrier function [38]. (H) Nanoscopy of dendritic spine dynamics in the brain of a living mouse [25]. All images were used with permission of the publishers.