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Figure 8 | Cell Communication and Signaling

Figure 8

From: Gβγ-mediated activation of protein kinase D exhibits subunit specificity and requires Gβγ-responsive phospholipase Cβ isoforms

Figure 8

Requirement of Gβγ -responsive PLCβ 2/3 isoforms in SDF-1α -induced signaling activities in Jurkat T-cells and HeLa cells. (A) Jurkat T-cells were transfected with the vehicle control (RNase free water), scrambled siRNA (siCon), siPLCβ1, or siPLCβ2 and siPLCβ3 oligonucleotides for 72 h prior to chemotactic assay upon SDF-1α treatment. Cells lysates were subjected to Western blot analysis with specific antibody against various PLCβ isoforms. β-actin was used as loading control. *Simultaneous knockdown the Gβγ-responsive PLCβ2 and PLCβ3 isoforms resulted in significant suppression of SDF-1α-induced chemotaxis. (B) HeLa cells subjected to similar PLCβ knockdown profiles were examined for SDF-1α-induced PKD activation, the levels of phospho-PKD1 in cell lysates were detected by Western blot analysis. The fold-induction represents the ratio of band intensities (phospho-PKD1) with respect to the basal (1.00) of the control group. Similar results were obtained in two independent experiments.

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