Figure 6From: Gβγ-mediated activation of protein kinase D exhibits subunit specificity and requires Gβγ-responsive phospholipase Cβ isoformsGβ 1 interacts with PKD1 in the presence of specific Gγ subunits and PLCβ 2 . HEK293 cells were transiently transfected with pcDNA3, PLCβ2, and different Gβγ combinations as indicated, followed by serum starvation. Cell lysates were subjected to immunoprecipitation by FLAG-tagged affinity gel and SDS-PAGE to resolve for proteins bound to FLAG-Gβ1. Black lines represent positions at which images from the same blot but on different lanes were merged.Back to article page