Figure 4

Stimulation of Gq/Gi-coupled receptors activates PKD1 in HeLa cells. HeLa cells were serum starved, followed by treatment with (A) 100 nM bradykinin (BK) or 10 μM histamine (His); (B) 10 μM isoproterenol (ISO) or 100 nM glucagon-like peptide (GLP); (C) 10 μM UK14304 (UK14) or 100 nM stromal cell-derived factor-1 (SDF-1) for the indicated times. Cell lysates were detected for phospho-PKD, PKD, phospho-ERK and ERK with their respective anti-sera.