Domain mapping of TIMAP-RACK1 interaction. (A): GST-TIMAP pull-down of endogenous RACK1. GST, recombinant GST-TIMAP WT or additional GST-TIMAP fragments (depicted in the upper part of panel A) were loaded onto glutathione-Sepharose as described in Materials and Methods. The immobilized protein samples were incubated with BPAEC lysate. Western blot of the pull-down eluates probed with anti-RACK1 antibody is shown. CL: total cell lysate. (B): GST-RACK1 pull-down of endogenous TIMAP. GST, recombinant RACK1 WT or GST-RACK1 fragments (depicted in the upper part of panel B) were tested in pull-down assay. The immobilized samples were incubated with BPAEC lysate. Western blot of the pull-down eluates was probed with anti-TIMAP antibody. Representative data of at least 3 independent experiments are shown.